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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in several tumors, which results in chromosome instability, and so could contribute to tumorigenesis. This protein was uncovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital position in endorsing mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually explained. Two more splice variants ended up described but specific nucleotide sequence facts is not really but out there.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified because of the antibody.
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This guideline outlines additional possible optimization of mobile and immunoassay parameters to make certain the best possible outcomes are...
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This guidebook outlines more possible optimization of cellular and immunoassay parameters to make sure the very best success are...
Antibodies sure to the blocking peptide now not bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody alone, you can see which staining is precise.
Focus on Data Cyclin E belongs for the very conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and functions to be a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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